ABSTRACT
Objective To investigate the effects of glutamine (Gln) on the p38MAPK activity and IL‐8 expression in periph‐eral blood mononuclear cells(PBMC) of the patients with chronic obstructive pulmonary disease(COPD) .Methods Thirty‐two pa‐tients with acute exacerbation of COPD (AECOPD ) were chosen as the research subjects(AECOPD group) .Then the patients con‐verting to the stable stage of COPD after treatment were set as the SCOPD group .PBMC of each group were extracted and conduc‐ted the Gln intervention .At the same time 16 healthy people were selected as the healthy control group .The real‐time PCR(RT‐PCR) was used to detect the levels of p38MAPK and IL‐8 expression in PBMC among the five groups .Results (1)The expressions of p38MAPK and IL‐8 in the AECOPD blank control group and the SCOPD blank control group were both higher than those in the healthy control group (P<0 .01 or P<0 .05) ,while the expression levels of p38MAPK and IL‐8 in patients with AECOPD was higher than those of patients with SCOPD ,the difference was statistically significant (P< 0 .05) .(2)The expression levels of p38MAPK and IL‐8 in the AECOPD Gln group were lower than those in the AECOPD blank control group (P<0 .01);the expres‐sion levels of p38MAPK and IL‐8 in the SCOPD Gln group were lower than those in the SCOPD blank control group (P<0 .05) . Conclusion Gln could inhibit the p38MAPK pathway activation in inflammatory cells of COPD patients and reduce the expression level of IL‐8 .
ABSTRACT
[Objective] To investigate the effect of salvianolate injection on the levels of Serum interleukin-1β(IL-1β)and interleukin-18(IL-18) in acute exacerbated chronic obstructive pulmonary disease(AECOPD) patients. [Methods] Fifty-eight patients with acute exacerbated COPD from Respiratory Dep. of thrid Hospital Affiliated to Zhejiang Chinese Medical University, were randomly assigned to the treatment group(28 cases) and the control group(30 cases). Routine therapies for COPD were given to patients in the control group, while 20mg salvianolate injection was given to those in the treatment group by intravenous dripping, once daily for total 10 days. The levels of IL-18, IL-1β and blood gas analysis were evaluated before and 10 days after therapy. The levels of the cytokins were compared with 30 normal controls. [Result] There were no significant differences in the value of IL-1β,IL-18,arterial partial pressure of carbon dioxide(PaCO2),arterial partial pressure of oxygen(PaO2) before treatment between two groups. Compared with the blank control group, the levels of serum IL-1β,IL-18 in patient with AECOPD were increased significantly( P<0.01). When compared with before treatment and with the control group, the levels of IL-1β,IL-18,PaCO2 decreased more significantly in the treatment group after treatment.The PaO2 in treatment group was higher than in control group. There were significant differences between two groups.[Conclusions] Salvianolate injection can reduce the secretion IL-1β, IL-18 in patient with AECOPD, which maybe through certain anti-inflammatory effects to improve clinical efficacy of COPD pa-tients.